Laboratory methods in phytopathology

Opterećenje: 15+15+0

Sadržaj predmeta: Lectures: 1. Basics and importance of laboratory methods in phytopathology. 2. Field collection of samples from diseased trees. Sample preparation and analysis. Preparation of nutrient media for the isolation and cultivation of pathogenic fungi, preparation of organ and/or tissue samples; procedures for obtaining and growing pure cultures of pathogenic fungi; sporulation methods. 3. Analyzes of isolated cultures of pathogenic fungi under a stereoloupe and light microscope. Making conclusions about the results of isolation, identification and inoculation, that is, a conclusion about the cause of a certain pathological phenomenon/disease. 4. Inoculation procedures and compliance with Koch's postulates for the purpose of confirming the pathogenicity of isolated fungi. 5. Passing conclusions on the results of isolation, identification and inoculation, i.e. a conclusion on the cause of a certain pathological phenomenon/disease. Exercises: 1. Preparation and analysis of organ and/or tissue samples under a stereoloupe and light microscope. 2. Field collection of samples from diseased trees, preparation of organ and/or tissue samples for the process of pathogen isolation from diseased cells/tissues. 3. Preparation of nutrient media for the isolation and cultivation of pathogenic fungi; preparation of organ and/or tissue samples for the process of isolating pathogens from diseased cells/tissues; procedures for obtaining and growing pure cultures of pathogenic fungi; sporulation methods. 4. The process of extracting the cellular DNA of the isolated pathogen and multiplying the target sequences by the PCR method; analysis of results by electrophoresis on agarose gel and sequencing.